Mice were killed at day 6 after tumor implantation, and their livers were snap frozen in optimal cutting temperature embedding compound for cryo-sectioning and IF analysis. Immunofluorescence, confocal microscopy, and image quantification. Please review our privacy policy. Arterioscler Thromb Vasc Biol J Biol Chem Li X, Eriksson U. Nitric oxide regulates tumor cell cross-talk with stromal cells in the tumor microenvironment of the liver.

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Quiescent HSCs were negative for both proteins arrowheads.

Semin Oncol 38Suppl 1: Author information Article notes Copyright and License information Disclaimer. Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover.

Am J Pathol Cytokine Growth Factor Rev Selective upregulation of platelet-derived growth factor alpha receptors by transforming growth factor beta in scleroderma fibroblasts.

Silencing of the transforming growth factor-beta TGFbeta receptor II by Kruppel-like factor 14 underscores the importance of a negative feedback mechanism in TGFbeta signaling. Human hepatic stellate cell lines, LX-1 and LX Mechanisms of TGF-beta signaling from cell membrane to the nucleus.

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Shi Y, Massague J. This article has been cited by other articles in PMC.

GAPDH was used as an internal control. T, tumor; S, stroma. HT29 human colorectal cancer cells were implanted into the livers of SCID mice by portal vein injection. Boyden chamber assay and cell proliferation assay.

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Ligand-dependent and -independent transforming growth factor-beta receptor recycling regulated by clathrin-mediated endocytosis and Rab Mechanisms of hepatic fibrogenesis. Densitometry of WB is shown on the right. Rheumatology 47Suppl 5: More than six representative cells in each group were selected for quantification and statistical analysis. Subsequently, the receptor complexes are internalized and sorted into early endosomes, where the receptors interact with and phosphorylate SMADs v759 J Exp Med Data represent multiple replicates.

Signal transduction in hepatic stellate cells.

Immunofluorescence, confocal microscopy, and image quantification. To assess the role of the conditioned media on HT29 cell migration, 15, tumor cells were seeded into each upper well of a Boyden chamber F759, Gaithersburg, MDand basal medium or conditioned medium was added into each lower chamber as a chemoattractant.

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Data represent 3 repeats with similar results. Densitometric analysis of WB blots was done using the Image J software. Role of PDGF in fibrotic diseases and systemic sclerosis.

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These 2 proteins colocalized at the plasma membrane arrowheads and in intracellular vesicles arrows. Differential properties of current tyrosine kinase inhibitors in gastrointestinal stromal tumors. J Biol Chem Next, the same siz IF technique was performed on liver biopsies of patients with colorectal cancer that were obtained from a Mayo Clinic tissue collection Cell nuclei were counterstained by Hoechst dye blue.